Structural Basis of Robo Proline-rich Motif Recognition by the Srgap1 Sh3 Domain in the Slit-robo Signaling Pathway
نویسندگان
چکیده
STRUCTURAL BASIS OF ROBO PROLINE-RICH MOTIF RECOGNITION BY THE SRGAP1 SH3 DOMAIN IN THE SLIT-ROBO SIGNALING PATHWAY Xiaofeng Li, Yushu Chen, Yiwei Liu, Jia Gao, Feng Gao, Mark Bartlam, Jane Y. Wu & Zihe Rao 1 “Tsinghua-IBP Joint Research Group for Structural Biology”, Tsinghua University, Beijing 100084, China. 2 National Laboratory of Biomacromolecules, Institute of Biophysics (IBP), Chinese Academy of Sciences, Beijing 100101, China. Department of Neurology; Center of Genetic Medicine, Lurie Cancer Center; Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA. Running title: Structure of the srGAP1 SH3 domain. ‡ Address correspondence to Jane Y. Wu, Tel: 312-503-0684, Fax: 312-503-5603, Email: [email protected]; or Zihe Rao, Tel: 86-10-62771493, Fax: 86-10-62773145, E-mail: [email protected].
منابع مشابه
Signal Transduction in Neuronal Migration Roles of GTPase Activating Proteins and the Small GTPase Cdc42 in the Slit-Robo Pathway
The Slit protein guides neuronal and leukocyte migration through the transmembrane receptor Roundabout (Robo). We report here that the intracellular domain of Robo interacts with a novel family of Rho GTPase activating proteins (GAPs). Two of the Slit-Robo GAPs (srGAPs) are expressed in regions responsive to Slit. Slit increased srGAP1-Robo1 interaction and inactivated Cdc42. A dominant negativ...
متن کاملA molecular mechanism for the heparan sulfate dependence of slit-robo signaling.
Slit is a large secreted protein that provides important guidance cues in the developing nervous system and in other organs. Signaling by Slit requires two receptors, Robo transmembrane proteins and heparan sulfate (HS) proteoglycans. How HS controls Slit-Robo signaling is unclear. Here we show that the second leucine-rich repeat domain (D2) of Slit, which mediates binding to Robo receptors, al...
متن کاملThe Inverse F-BAR Domain Protein srGAP2 Acts through srGAP3 to Modulate Neuronal Differentiation and Neurite Outgrowth of Mouse Neuroblastoma Cells
The inverse F-BAR (IF-BAR) domain proteins srGAP1, srGAP2 and srGAP3 are implicated in neuronal development and may be linked to mental retardation, schizophrenia and seizure. A partially overlapping expression pattern and highly similar protein structures indicate a functional redundancy of srGAPs in neuronal development. Our previous study suggests that srGAP3 negatively regulates neuronal di...
متن کاملStructural insight into Slit-Robo signalling.
Drosophila Slit and its vertebrate orthologues Slit1-Slit3 are secreted glycoproteins that play important roles in the development of the nervous system and other organs. Human Slits are also involved in a number of pathological situations, such as cancer and inflammation. Slits exert their effects by activating receptors of the Robo (Roundabout) family, which resemble cell adhesion molecules i...
متن کاملRepellent signaling by Slit requires the leucine-rich repeats.
Slit is a repellent axon guidance cue produced by the midline glia in Drosophila that is required to regulate the formation of contralateral projections and the lateral position of longitudinal tracts. Four sequence motifs comprise the structure of Slit: a leucine-rich repeat (LRR), epidermal growth factor-like (EGF) repeats, a laminin-like globular (G)-domain, and a cysteine domain. Here we de...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006